Developing a transposon tagging system to isolate rust-resistance genes from flax
Identifieur interne : 001905 ( Main/Exploration ); précédent : 001904; suivant : 001906Developing a transposon tagging system to isolate rust-resistance genes from flax
Auteurs : J. G. Ellis [Australie] ; E. J. Finnegan [Australie] ; G. J. Lawrence [Australie]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1992.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Teeft :
- Acci digestion, Agrobacterium, Agrobacterium tumefaciens, Bamhi site, Callus, Callus tissue, Cdna copy, Copy number, Embryo development, Excision, Excision band, Flax, Flax callus, Flax cotyledons, Flax plants, Flax rust, Fragment, Gene, Germline, Hindiii, Hoshangabad, Hybrid progeny, Hybrid seed, Increase transposition, Junction fragments, Maize, Maize element activator, Maize transposable element, Maize transposable elements, Nonparental copies, Nonparental fragment, Parental copies, Plant cells, Primary infection, Probe, Probe locations, Proc natl acad, Progeny, Progeny analysis, Progeny plants, Promoter, Promoter probe, Promoter probes, Reporter gene, Resistance gene, Resistance genes, Restriction fragments, Schell, Selectable marker, Selfed progeny, Similar vectors, Smallest fragment, Solanaceous plants, Southern analysis, Transgenic, Transgenic plants, Transposable, Transposable elements, Transposase, Transposase gene, Transposition, Transposition frequency, Transposon.
Abstract
Summary: A line of flax, homozygous for four genes controlling resistance to flax rust, was transformed with T-DNA vectors carrying the maize transposable elements Ac and Ds to assess whether transposition frequency would be high enough to allow transposon tagging of the resistance genes. Transposition was much less frequent in flax than in Solanaceous hosts such as tobacco, tomato and potato. Transposition frequency in callus tissue, but not in plants, was increased by modifications to the transposase gene of Ac. Transactivation of the excision of a Ds element was achieved by expressing a cDNA copy of the Ac transposase gene from the Agrobacterium T-DNA 2′ promoter. Progeny of three plants transformed with Ac and 15 plants transformed with Ds and the transposase gene, were examined for transposition occurring in the absence of selection. Transposition was observed in the descendants of only one plant which contained at least nine copies of Ac. Newly transposed Ac elements were observed in 25–30% of the progeny of some members of this family and one active Ac element was located 28.8 (SE=6.3) map units from the L 6 rust-resistance gene. This family will be potentially useful in our resistance gene tagging program.
Url:
DOI: 10.1007/BF00223843
Affiliations:
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Le document en format XML
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Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher ref="https://scientific-publisher.data.istex.fr/ark:/67375/H02-SWLMH5L1-1">Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1992">1992</date><biblScope unit="vol" from="85" to="85">85</biblScope><biblScope unit="issue" from="1" to="1">1</biblScope><biblScope unit="page" from="46">46</biblScope><biblScope unit="page" to="54">54</biblScope></imprint><idno type="ISSN">0040-5752</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungus resistance</term><term>Inheritance</term><term>Investigation method</term><term>Linum usitatissimum</term><term>Melampsora lini</term><term>Transposon</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Déterminisme génétique</term><term>Linum usitatissimum</term><term>Melampsora lini</term><term>Méthode étude</term><term>Résistance champignon</term><term>Transposon</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acci digestion</term><term>Agrobacterium</term><term>Agrobacterium tumefaciens</term><term>Bamhi site</term><term>Callus</term><term>Callus tissue</term><term>Cdna copy</term><term>Copy number</term><term>Embryo development</term><term>Excision</term><term>Excision band</term><term>Flax</term><term>Flax callus</term><term>Flax cotyledons</term><term>Flax plants</term><term>Flax rust</term><term>Fragment</term><term>Gene</term><term>Germline</term><term>Hindiii</term><term>Hoshangabad</term><term>Hybrid progeny</term><term>Hybrid seed</term><term>Increase transposition</term><term>Junction fragments</term><term>Maize</term><term>Maize element activator</term><term>Maize transposable element</term><term>Maize transposable elements</term><term>Nonparental copies</term><term>Nonparental fragment</term><term>Parental copies</term><term>Plant cells</term><term>Primary infection</term><term>Probe</term><term>Probe locations</term><term>Proc natl acad</term><term>Progeny</term><term>Progeny analysis</term><term>Progeny plants</term><term>Promoter</term><term>Promoter probe</term><term>Promoter probes</term><term>Reporter gene</term><term>Resistance gene</term><term>Resistance genes</term><term>Restriction fragments</term><term>Schell</term><term>Selectable marker</term><term>Selfed progeny</term><term>Similar vectors</term><term>Smallest fragment</term><term>Solanaceous plants</term><term>Southern analysis</term><term>Transgenic</term><term>Transgenic plants</term><term>Transposable</term><term>Transposable elements</term><term>Transposase</term><term>Transposase gene</term><term>Transposition</term><term>Transposition frequency</term><term>Transposon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: A line of flax, homozygous for four genes controlling resistance to flax rust, was transformed with T-DNA vectors carrying the maize transposable elements Ac and Ds to assess whether transposition frequency would be high enough to allow transposon tagging of the resistance genes. Transposition was much less frequent in flax than in Solanaceous hosts such as tobacco, tomato and potato. Transposition frequency in callus tissue, but not in plants, was increased by modifications to the transposase gene of Ac. Transactivation of the excision of a Ds element was achieved by expressing a cDNA copy of the Ac transposase gene from the Agrobacterium T-DNA 2′ promoter. Progeny of three plants transformed with Ac and 15 plants transformed with Ds and the transposase gene, were examined for transposition occurring in the absence of selection. Transposition was observed in the descendants of only one plant which contained at least nine copies of Ac. Newly transposed Ac elements were observed in 25–30% of the progeny of some members of this family and one active Ac element was located 28.8 (SE=6.3) map units from the L 6 rust-resistance gene. This family will be potentially useful in our resistance gene tagging program.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Ellis, J G" sort="Ellis, J G" uniqKey="Ellis J" first="J. G." last="Ellis">J. G. Ellis</name></noRegion><name sortKey="Finnegan, E J" sort="Finnegan, E J" uniqKey="Finnegan E" first="E. J." last="Finnegan">E. J. Finnegan</name><name sortKey="Lawrence, G J" sort="Lawrence, G J" uniqKey="Lawrence G" first="G. J." last="Lawrence">G. J. Lawrence</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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